重组白细胞介素2对乙型肝炎患者自然杀伤活性的调节

IL_2REGULATION OF NK CELL ACTIVITY IN PATIENTS WITH HEPATITIS B

应用^(125)I释放法同时观察了38例乙型病毒性肝炎病人及22例正常人外周血单个核细胞经重组白细胞介素2作用前后自然杀伤细胞活性,并对其活性改变及调节机制进行探讨。发现慢性活动性肝炎和重症肝炎病人血清中存在某种抑制因子,该抑制因子能抑制白细胞介素2活性或阻断其作用的发挥。

The natural killer (NK) cell activity in vitro and the regulating effect of recombinant interleukin 2 (r-IL2) on NK in 38 cases of viral hepatitis B was investigated by calculating125I-UdR released from the labelled target K562 cells.Of the 38 patients, 22 were chronic active hepatitis (CAH) and 16 severe hepatitis (SH) .The NK cell activity in patients with CAH and SH was significantly lower than that of the normal subjects (P< 0.01) .When r-IL2 was added into the culture media the NK cell activity was augmented in both CAH and SH (P<0.01), but still significantly below the normal level; in addition with the existence of patient sera, IL2 failed to augment the NK cell activity. It is indicated that there were certain IL2inhibitory factor in the patients' sera.