用气孔保卫细胞叶绿体计数法鉴定烟草染色体倍性方法初探

Research on the Method of Identify the Multiple Tobacco Chromosomes Using Count Stoma Protective Cell Chloroplast

经不同浓度秋水仙碱液加倍处理的烟草花粉植株 ,用叶片气孔保卫细胞叶绿体计数法鉴定染色体倍性。研究表明 :气孔保卫细胞叶绿体数平均值差异极显著 ,单倍体 95 %以上的叶绿体数在 14个以下 ,双倍体 95 %以上的叶绿体数则在 14个以上。经开花结实验证其准确率达 91% ,且展开到第

The tobacco pollen plants which multiply treated by the different concentration colchichnes were identified the multiple chromosomes using count the leave stoma protective cell chloroplast. The results indicated that the discrepancy of the average chloroplast number was extemely prominet more than 95% chloroplast number of the haploid were below 14, and more than 95% chloroplast number of the diploid were above 14. The degree of accuracy is 19%, which tested and verified by flowering and seeding, and it could be identified when vegetative development up to the fifth leaf as well.