长期小剂量应用关木通对部分肾切除大鼠肾脏的影响

Influence of Long-Term Use of Low Dose Caulis Aristolochiae Manshuriensis on Partial Nephrectomized Rats

目的 :了解长期小剂量应用关木通是否可加重慢性肾衰竭大鼠肾脏损害。方法 :采用 5 /6肾切除方法复制大鼠慢性肾衰竭模型 ,第 1组给予与药典剂量相当 (1g/kg)的关木通煎剂 ,第 2组给予关木通3g/kg ,第 3组给予相同量的自来水 ,均每天灌胃 1次 ,共 8周 ,观察血清肌酐、尿素氮、尿蛋白含量以及肾脏形态变化。结果 :8周后 ,第 2组血清肌酐、尿素氮和尿蛋白排泄量显著高于第 3组〔分别为 (16 5 0± 15 6 )和 (10 9 8± 10 0 ) μmol/L ,(2 7 8± 3 6 )和 (18 7± 2 5 )mmol/L ,(6 8 2± 10 1)和 (44 6± 8 5 )mg/2 4h ,P值均 <0 0 5〕 ,肾间质病变和肾小球硬化程度也较重。结论 :慢性肾衰竭大鼠对小剂量关木通的肾脏毒性作用的易感性增加 。

Objective: To study the effect of long term use of low dose Caulis Aristolochiae Manshuriensis (CAM) on the deterioration of chronic renal failure rats. Methods: The 5/6 nephrectomized Wistar rats were taken as animal model of chronic renal failure, which were divided into 3 groups. Group A was treated with 1 g/kg CAM decoction, the dose equivalent to that defined in the pharmacopoeia. Group B was treated with 3 g/kg CAM decoction and Group C treated with equal volume of tap water. Medication was given once per day by gastrogavage in all the three groups for 8 weeks. The serum creatinine, urea nitrogen, urinary protein content and morphological changes of kidney were observed. Results: After 8 weeks treatment, levels of serum creatinine, urea nitrogen, urinary protein excretion in Group B were higher than those in Group C significantly, they were 165.0±15.6 μmol/L vs 109.8±10.0 μmol/L, 27.8±3.6 mmol/L vs 18.7±2.5mmol/L and 68.2±10.1mg/24 hrs vs 44.6±8.5 mg/24 hrs, respectively, all P<0 05. The pathological changes of renal mesenchyme and degree of glomerulosclerosis were also heavier in Group B. Conclusion: The susceptibility of chronic renal failure rats to the nephrotoxicity of CAM increases in long term use of low dose CAM which could accelerate the deterioration of renal impairment in the model rats.