胃癌及癌前病变患者血清中MG_7抗原的检测及临床意义的探讨

Detect and clinical explore of serous MG_7Ag in gastric cancer and precancerous cancer patients

目的:探讨胃癌及癌前病变患者血清中MG7抗原表达及其临床意义。方法:实验分二组,实验组为胃癌组90例。其中高分化腺癌30例,低分化腺癌46例,印戒细胞癌14例;胃粘膜上皮不典型增生(中、重度)60例。对照组为胃良性疾病30例,其中慢性浅表性胃炎10例,慢性萎缩性胃炎10例,胃溃疡10例;应用聚合酶链反应(PCR),常规操作9个步骤共30个循环,每一循环包括DNA变性,引物退火及链的延伸。结果:90例胃癌患者血清呈阳性者78例(86.6%),60例胃黏膜不典型增生患者血清呈阳性者10例(16.7%);30例对照组胃良性疾病患者血清呈阳性4例(13.3%)。胃癌组与胃良性疾病组对比差异显著(P<0.01),胃癌根治术后较根治术前血清MG7抗原阳性表达明显下降,胃黏膜不典型增生阳性者10例,进行跟踪随诊,阳性组癌变高于阴性组,有统计学意义(P<0.05)。结论:胃癌单克隆抗体相关抗原MG7这一组新的中性糖脂相关抗原对胃癌诊断有其特异性,可作为胃癌诊断有效指标,可以达到早期诊断目的,提高胃癌诊断率。

ve In the experiment serous antigen MG7 of stomach carcinoma patients was detected by PCR technique to judge if is valuable in the diagnosis of stomach carcinoma. Method PCR technique was used to detect serous monoclonal antibody carcino-ma, 30 cases of benign diseases, 60 cases of atypical hyperplasia of gastric mucosal epithelia. Results Positive rate of MG7Ag in carcino-ma, benign disease and atypical hyperplasia groups are 86.6% , 13.3% and 16.7 respectively. Positive expression of MG7Ag in stomach carcinoma was significantly (P<0.05).Positive expression in carcinoma group before radical operation. MG7Ag was detected in different types of stomach cancer by different immune labels.significant difference was shown (P<0.05).No significant difference was shown in expression of MG7Ag in groups of atypical hyperplasia. Conclusion This indicates that MG7Ag detecting is valuable in diagnosis of stom-ach carcinoma, patients of atypical hyperplasia with positive expression of MGyAg are high rise group of stomach cancer and should be fol-lowed up strictly, also can improve diagnostic rate of stomach carcinoma.