氯苄四氢小檗碱对抗H_2O_2引起无血清培养的血管内皮细胞凋亡及坏死

Inhibition of chlorobenzyl-tetrahydroberberine on the H_2O_2-induced apoptosis and proliferation of endothelial cells

目的 :研究氯苄四氢小檗碱对过氧化氢(H2 O2 )引起无血清培养的小牛主动脉血管内皮细胞的凋亡、增殖、坏死的影响。方法 :通过体外细胞培养 ,以噻唑兰 (MTT)法测定细胞存活率 ;用流式细胞术检测细胞DNA含量及凋亡细胞百分率、增殖率 ;DNA琼脂糖凝胶电泳法观察细胞凋亡过程中DNA断裂程度。结果 :H2 O2 诱导无血清培养的内皮细胞凋亡 ,氯苄四氢小檗碱可显著降低内皮肌细胞中凋亡细胞百分率 ,并抑制其增殖 ,也减少凋亡细胞DNA断裂 ,同时也减少其引起的平滑肌细胞坏死。结论 :氯苄四氢小檗碱可对抗H2 O2 引起的无血清培养的血管平滑肌细胞凋亡。

AIM: To examine the inhibition of chlorobenzyl tetrahydroberberine(BTHB) on the H 2O 2 induced apoptosis and proliferation of endothelial cells(ECs) in the serum deprivation medium. METHODS: The DNA content and percentage of apoptosis were measured by flow cytometry; the DNA fragmentation was analyzed by agarose gel electrophorsis; the viability percentage of cells was measured using MTT assay. RESULTS: The apoptosis and proliferation of ECs occured in serum free medium (18 h) containing H 2O 2. When the ECs had been treated with BTHB, the percentage of ECs apoptosis and proliferation was markedly decreased, and the degraded fragments of DNA obviously decreased. BTHB (10 -5 , 10 -6 , 10 -7 , 10 -8 mol·L -1 ) could partly protect cells from necrosis induced by H 2O 2. CONCLUSION: BTHB can prevent ECs from apoptosis, proliferation and necrosis induced by H 2O 2 in free serum medium.