摘要
目的 :研究氯苄四氢小檗碱对过氧化氢(H2 O2 )引起无血清培养的小牛主动脉血管内皮细胞的凋亡、增殖、坏死的影响。方法 :通过体外细胞培养 ,以噻唑兰 (MTT)法测定细胞存活率 ;用流式细胞术检测细胞DNA含量及凋亡细胞百分率、增殖率 ;DNA琼脂糖凝胶电泳法观察细胞凋亡过程中DNA断裂程度。结果 :H2 O2 诱导无血清培养的内皮细胞凋亡 ,氯苄四氢小檗碱可显著降低内皮肌细胞中凋亡细胞百分率 ,并抑制其增殖 ,也减少凋亡细胞DNA断裂 ,同时也减少其引起的平滑肌细胞坏死。结论 :氯苄四氢小檗碱可对抗H2 O2 引起的无血清培养的血管平滑肌细胞凋亡。
AIM: To examine the inhibition of chlorobenzyl tetrahydroberberine(BTHB) on the H 2O 2 induced apoptosis and proliferation of endothelial cells(ECs) in the serum deprivation medium. METHODS: The DNA content and percentage of apoptosis were measured by flow cytometry; the DNA fragmentation was analyzed by agarose gel electrophorsis; the viability percentage of cells was measured using MTT assay. RESULTS: The apoptosis and proliferation of ECs occured in serum free medium (18 h) containing H 2O 2. When the ECs had been treated with BTHB, the percentage of ECs apoptosis and proliferation was markedly decreased, and the degraded fragments of DNA obviously decreased. BTHB (10 -5 , 10 -6 , 10 -7 , 10 -8 mol·L -1 ) could partly protect cells from necrosis induced by H 2O 2. CONCLUSION: BTHB can prevent ECs from apoptosis, proliferation and necrosis induced by H 2O 2 in free serum medium.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2002年第3期200-204,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
过氧化氢
氯苄四氢小檗碱
主动脉
内皮细胞
增殖
凋亡
流式细胞术
凝胶电泳
BTHB
hydrogen peroxide
chlorobenzyl tetrahydroberberine
thoracic aorta endothlial cell
apoptosis
proliferation
flow cytometry
agarose gel electrophorsis