摘要
高速逆流色谱是一种新发展起来的无载体的液液分配色谱技术。它利用聚四氟乙烯(PTFE)螺旋管的方向性与高速行星式运动相结合,产生一种独特的流体动力学现象,使互不相溶的两相溶液在螺旋管中充分混合,顺序传递,导致样品中各个组分由于分配系数的差别而有效的分离。本文介绍了国产的高速逆流色谱仪及应用该仪器制备分离云南红豆杉中结构相近的三种紫杉烷类二萜及二萜生物碱。
high speed countercurrent chromatography (HSCCC)based on the fundamental principle of liquid-liquid partition is a newly developed technique for isolation. It utilizes a particular combination of coil orientation and planetary motion to produce a unique hydrodynamic phenomenon in the unilateral phase distribution of two immiscible solvents in a coiled column. In recent years HSCCC has gained increasing popularity in the isolation of bioactive natural products.This paper reports its application in the prepartive separation of taxol (1), cephalomannine (2)and baccatin-Ⅲ (3) from the alcoholic extract of Taxus yunnanensis. These 3 ingredients are first obtained from this plant.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第7期510-514,共5页
Acta Pharmaceutica Sinica
关键词
高速逆流色谱
云南红豆杉
分离
High speed countercurrent chromatography
Taxus yunnanensis
Taxanediterpenoids and diterpene alkaloids
