T细胞因子4基因表达与肝癌侵袭转移的关系

Relationship between hTcf-4 gene expression and liver cancer metastasis.

目的 研究人T细胞因子 4基因 (hTcf- 4)在原发性肝癌及肝癌细胞株中表达情况 ,以探讨hTcf- 4基因与肝癌发生发展的关系。方法 用逆转录 -聚合酶链反应 (RT -PCR) ,检测了 32例原发性肝癌组织、癌旁组织及 5株肝癌细胞株中hTcf- 4基因表达。结果 hTcf- 4mRNA在癌旁组织中的表达率为 71 9% ,而在癌组织中的表达率达 90 6 %。且癌组织中的表达水平明显高于癌旁组织 (P <0 0 0 1)。同时在肿瘤包膜不完整及有转移的癌组织中该基因表达明显增高。不同细胞株中均有hTcf- 4基因表达。肝癌细胞株中表达水平高于正常肝细胞株 (P <0 0 0 1) ,而高转移肝癌细胞株MHCC97中表达水平又高于低转移肝癌细胞株 (P <0 0 5 )。结论 hTcf- 4基因表达增高与肝癌发生发展及侵袭转移有关 ,提示hTcf- 4可能成为协助肝癌诊断、判断预后及疗效评价的指标。

Objective To study the expression of hTcf-4 gene in hepatocellular carcinoma(HCC) and liver cancer cell lines. Methods Reverse Transcription-Polymerase Chain Reaction(RT-PCR)method was used to detect the expression of hTcf-4 mRNA in 32 HCC samples, the corresponding peripheral tissues and liver cancer cell lines. Results The expression rates of hTcf-4 mRNA in HCCs and the corresponding peripheral tissues were 90.6% and 71.9%, respectively. The expression level of hTcf-4 gene in HCCs was much higher than that in the peripheral tissues( P <0.001). And all cell lines detected had hTcf-4 gene expression. The expression level of hTcf-4 gene in liver cancer cell lines was much higher than that in normal liver cell line( P <0.001). Furthermore, the expression level in highly metastatic liver cancer cell line (MHCCC97) was higher than that in low metastatic liver cancer cell lines( P <0.05). Conclusion The over-expression of hTcf-4 gene maybe related to the development and invasion of liver cancer.