胰腺癌组织增殖细胞核抗原的测定及其临床意义

Detection of PCNA in pancreatic adenocarcinoma and its clinical significance.

目的 研究胰腺癌组织中增殖细胞核抗原 (PCNA)的表达与临床生物学行为之间的关系。方法 应用免疫组织化学ABC法对 39例存档胰腺癌标本和 8例慢性胰腺炎标本进行PCNA的定量分析。结果 39例胰腺癌组织中PCNA增殖指数 (LI)为 2 7 5 4± 16 42 ,而 8例胰腺炎组织中未发现有PCNA的阳性表达 ,PCAN增殖指数与患者的年龄、性别及肿瘤的部位无关 (P >0 0 5 ) ,而与肿瘤的大小、组织学分级、临床分期及淋巴结转移密切相关。结论 PCNA是反映胰腺癌生物学行为和预后的重要指标 ,高表达提示肿瘤的恶性程度高 ,预后不良。

Objective To investigate the expression of PCNA and the clinicobiological behavior in human pancreatic adenocarcinoma. Methods The expression of PCNA in 39 cases of human pancreatic adenocarcinoma and 8 cases of chronic pancreatitis were tested by immunohistochemistry of avidin-biotin comples. Results In the 39 cases of pancreatic ductal adenocarcinoma, the PCNA LI was 27.54±16.42,but no positive expression was found in chonic pancreatitis. The PCNA labeling index with no relation to sex, age, tumor location, however, there were significant differences between PCNA LI and tumor size, histological grade, clinical stage, lymph node status. Conclusion The expression of PCNA is an important marker reflecting the biological behaviors and prognosis of pancreatic cacinoma, the higher PCNA LI suggests high maligance and poor prognosis.