超临界流体色谱法测定三七及云南白药中人参二醇和人参三醇的含量

DETERMINATION OF ERGOSTEROL IN CORDYCEPS SINENSIS AND CORDYCEPS BLACK-BONE CHICKEN CAPSULES BY HPLC

本文将样品直接皂化,环己烷提取,用分配柱和吸附柱进行提取液的净化和浓缩,HPLC法测定了冬虫夏草及虫草乌鸡胶丸中麦角甾醇的含量,为虫草及其制剂中麦角甾醇的含量测定建立了简便可行的方法。

Capillary supercritical fluid chromatography (SFC)was developed for the determination of panaxadiol and panaxatriol in Radix notoginseng and Yunnan Baiyao. 0.1 g Radix notoginseng powder or 0.5 g Yunnan Baiyao was mixed with 10 ml 15% H_2SO_4 ethanol- water (1:1 )solution, adding 1 mg cholesterol as internal standard. The mixture was refluxed for 4 h, then adding 15 ml 15% NaOH solution, refluxed for 0. 5 h.The mixture was extracted 3 times with 10 ml portions of cyclohexane. The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by SFC. The proposed method is sensitive, accurate, precise, simple and rapid;all the process can be done in 8 h.