摘要
本文将样品直接皂化,环己烷提取,用分配柱和吸附柱进行提取液的净化和浓缩,HPLC法测定了冬虫夏草及虫草乌鸡胶丸中麦角甾醇的含量,为虫草及其制剂中麦角甾醇的含量测定建立了简便可行的方法。
A high performance liquid chromatographic method (HPLC)was developed for the determination of ergosterol in Cordyceps sinensis and Cordyceps black-bone chicken capsules. The sample was mixed with 0.5 ml of saturated KOH solution and 5 ml of ethanol, and refluxed for 1h. The saponified mixture was diluted with 5ml of 10% NaCl solution and extracted 3 times with 5 ml portions of cyclohexane. The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by HPLC. The other conponents of the Cordyceps black- bone chicken capsules have no interference to the determination of ergosterol. The proposed method is rapid, precise, accurate and sensitive. The whole process can be done in 2h.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第10期768-771,共4页
Acta Pharmaceutica Sinica
关键词
冬虫夏草
麦角甾醇
蛹虫草
HPLC
Ergosterol
Cordyceps sinensis
Cordyceps militaris
Cultured Cordyceps
Cordyceps black- bone chicken capsule
