非相关成分参比一倍率导数光谱法测定制剂中甲氧苄啶和磺胺甲(口恶)唑

DETERMINATION OF TRIMETHOPRIM AND SULFAMETHOXAZOLE IN PHARMACEUTICAL PREPARATIONS BY NON-RELATIVE COMPONENT REFERENCE-MULTIPLIER DERIVATIVE SPECTROPHOTOMETRY

提出了非相关成分参比一倍率导数光谱法用于测定混合组分体系的基本原理和实验方法。本法可用于待测成分和非相关成分导数光谱严重重叠且待测成分含量较低时的不经分离直接测定。试用本法消除了甲氧苄啶(TMP)和磺胺甲嚼唑(SMZ)及附加剂之间的相互干扰,从而测定了部分制剂中的TMP:用“零交法”测定了SMZ,平均回收率分别为:102.5±1.63%(CV)和100.3±0.99%(CV)。

This paper provides the basic principle and experimental technique of non-relative component reference-multiplier derivative spectrophotometry. The procedure overcomes the problem of overlapping in derivative spectrophotometry and quantitative analysis of lower content component in mixture can be done without separation. This method was investigated to assay the contents of trimethoprim (TMP) and used to assay the zero crossing derivative spectrophotometry for the assay of sulfamethoxazole (SMZ) in pharmaceutical preparations. The average recoveries of TMP and SMZ were 102.5± 1.63% (CV) and 100.3±0.99% (CV) respectively. The results show that it not only can effectively remove the interference from each other, but also give a higher sensitivity and accuracy.