三七细胞大量培养物中主要药用成分皂甙的分离和鉴定

SEPARATION AND IDENTIFICATION OF MAIN MEDICINAL SAPONIN COMPONENTS FROM MASS CELL CULTURES OF PANAX NOTOGINSENG (BURK) F.H. CHEN

植物细胞大量培养技术工业化生产次级代谢产物,与栽培植物比较,具有不占用良田、不受地区、季节、气候的影响,无农药残留,不影响出口及药用,很少受市场波动的影响,有利于提高生产率降低成本,提高有效成分含量和获得新的有用物质等优点。三七Panax notoginseng(Burk)F.H.Chen为云南特产的名贵中药。

Five main saponins were separated from the cell cultures of Panax notoginseng (Burk) F. H. Chen. They were sanchinoside Rb_1, Re, Rl, Rg_1 and Rh_1 identified by TLC, HPLC, IR, M. P., ^(13)CNMR and EI- MS. Saponin components of the cell cultures were almost the same as those of the cultivated plants. But the content of saponins was different between the cell cultures and the cultivated plants. Saponin extraction and separation procedure suitable for the cell cultures has to be different from that for the cultivated plants.