湖北贝母属植物化学成分的研究——ⅩⅢ湖贝甙的分离与结构解析

STUDIES ON CHEMICAL CONSTITUENTS OF FRITILLARIA IN HUBEI. ⅫⅠ ISOLATION AND STRUCTURE ELUCIDATION OF HUPEHEMONOSIDE

从湖北贝母Fritillaria hupehensis Hsiao et K.C.Hsia经干燥的鳞茎中分离出Ⅰ～Ⅳ4个生物碱,经理化常数测定和波谱分析,并与标准品对照,鉴定碱Ⅰ,Ⅱ和Ⅲ分别为浙贝甲素(veimine)、浙贝乙素(Peiminine)和鄂贝新(ebeiensine),其中鄂贝新咱湖北贝母中首次得到.碱Ⅳ是一种微量生物碱甙,C_(33)H_(53)NO_8,mp206～208℃,定名为湖贝甙(hupehemonoside),经IR,MS,~1HNMR和^(13)CNMR解析,推定其结构为5α,14α,17β,22β-cevanine-20β-hydroxyl-6-oxo-3β-O-β-D-glucoside。

A new D/E cis-5-cevanine alkaloidal glucoside, C_(33)H_(53)NO_8, mp 206～208℃, named hupehemonoside, was isolated together with three known alkaloids, peimine, peiminine and ebeiensine from the fresh bulb of Fritillaria hupehensis Hsiao et K. C. Hsia.On the basis of the IR, ~1H-NMR, ^(13)C-MNR, FAB-MS and EI-MS of hupehemonoside, it has been established as 5α, 14α, 17β, 22β-cevanine-20β-hydroxyl - 3β- O - β - D- glucoside (Ⅳ).