摘要
紫草科紫草属植物新疆紫草Amebia euchroma(Rogle)Johnst和紫草Lithospermun erythrorhizon Sieb.et Zucc的根为传统中药。七十年代以来,国内外先后从不同产地的紫草中提取分离和测定了多种具有生理活性的萘醌(naphthoquinone)类化合物。这些成分有抗炎及抗肿瘤作用。长期以来,由于大量采挖紫草,其资源日趋减少。
A simple, sensitive and accurate method for the separation and determination of the naphthoquinones, deoxyshikonin (Ⅰ), acetylshikonin (Ⅱ) and β-β-dimethylacrylshikonin (Ⅲ) in tissue cultured Zicao is described.The sample solution was applied at a point 1 cm from the bottom edge of the TLC silica gel plate ( 10×15cm ). A mixture of n-hexane- acetone- chloroform-glacial acetic acid ( 13:0.3:0.2:0.05 ⅴ/ⅴ) was used as the developing solvent. The plate was saturated for 30 min and then devdoped twice for 15 cm using ascending technique. The spots were scraped off and extracted with CHCl_3, then determined spectrophotometrically.The standard curve was linear in the range of 0.65～2.25μg for the naphthoquinones.The method has been applied to the analysis of different samples. This method can be used for the quality control of naphthoquinones in tissue culture products.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第12期953-955,共3页
Acta Pharmaceutica Sinica
关键词
紫草
萘醌
测定
Zicao
Spectrophotometry
Naphthoquinones
TLC
Tissue culture
