摘要
本文以酶法制备新生大鼠脑细胞悬液,运用近年来发展起来的Fura-2技术,检测此神经细胞内游离钙(以下简写为[Ca^(2+)]i)及其变化。结果表明:在静息状态下,其[Ca^(2+)]i为240±5nmol/L。高钾去极化可使[Ca^(2+)]i成倍增加.钙拮抗剂verapamil和Ilifedipinc能阻断高钾升高[Ca^(2+)]i的作用。实验结果证明了所制备的神经细胞悬液的可用性及建立的Fura-2测定[Ca^(2+)]i方法的可靠性。
The intracellular free Ca^(2+) concentration was measured in freshly dissociated brain cells prepared from neonatal rats using the fluorescent Ca^(2+) indicator Fura- 2/AM. Cytosolic Ca^(2+) concentration of resting cells was calculated to be 240±5 nmol/L. Depolarization with high K^+ resulted in an over 100% increase in intracellular Ca^(2+) concentration, and this increase could be prevented or reversed by verapamil or nifedipine known to block voltage-sensitive Ca channels. These results suggest that the adoption of Fura- 2/AM method in freshly dissociated rat brain cells is a useful and relatively easily applicable technique for monitoring intracellular Ca^(2+) changes.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第12期890-894,共5页
Acta Pharmaceutica Sinica
