单克隆抗体C50 ^(99)Tc^m标记药盒的制备

A study of labeling of monoclonal antibody C50 with ^(99)Tc^m and using for radioimmunoimaging

目的 探讨99Tcm C5 0放免显像药盒的制备方法。方法 用 2 亚氨噻酚盐酸盐修饰C5 0 ,通过99Tcm 葡庚糖酸钠 (GH)转换 ,将99Tcm 标记在C5 0上。用HPLC法分析修饰抗体和标记抗体 ,用纸层析法测定标记率和胶体 ,并用免疫分析法测定标记抗体生物活性。裸鼠尾静脉注射抗体后进行显像。结果 C5 0标记率为 97% ,其中99Tcm 胶体含量为 4% ,修饰抗体 4℃下可保存 3个月 ,标记抗体有良好的稳定性和免疫活性。荷瘤裸鼠 2 4h肿瘤部位与四肢肌肉的放射性比值为 4.0 3。结论该制备方法高效、便捷 ,并可用于其他单抗放免显像药盒的制备。

Objective To provide an accurate and scientific method of radionuclide imaging with 99 Tc m-C50. Methods The labeled C50 was derived by iminothiolane and ligand exchange from 99 Tc mO(GH) -_2. HPLC was used to analyse the modified antibody and labeled antibody, labeling efficiency was measured by paper chromatography. Radioimmunoimaging was performed on human colon tumor bearing nude mice. Results The labeling efficiency of the labeled antibody was 97%, with 4% colloidal 99 Tc m in it; the in vitro competition test showed that 99 Tc m being bound up with the antibody at high affinity. The stability of the iminothiolane modified antibody could be kept at 4 ℃ for 3 months. The biodistribution study showed that the tumor radioactivity uptake at 24 h postinjection was the highest, the ratio of tumor to muscle was 4.03. Conclusion The labeling method applied to the preparation of 99 Tc m labeled antibody C50 was successful, and appears to be equally applicable for labeling other antibodies.