地黄脱毒技术研究

Study on the Technique of Rehmannia glutinosa Virus-free

从地黄种茎育出的无菌幼苗上切取下的茎尖,用组织培养方法可诱导成去病毒幼苗。本试验还研究出适宜地黄茎段苗消毒的消毒剂为0.05％HgCl_2。适宜于茎段苗生长的MS培养基的琼脂浓度为0.7％,pH为7。诱导茎尖成苗的培养基为MS+6-BA 0.5 mg/L。繁殖脱毒苗所用的MS培养基为1/4大量元素+1/2微量元素,并用1/2食用白砂糖代替蔗糖,可使快繁成本降低48.7％。

The stem tip from germ-free stem segment of Rehmannia glutinosa cultured in test tube can be induced into virus-free seedling.The experiment showed that the proper disinfectant for stem segments of Rehmannia glutinosa was 0.05% HgCl2.The seedings from stem segments grew better with MS in the concentration of agar 0.7% and pH7. The stem tips could be directly induced to seedlings by using MS + 6-BA(0.05 mg/L) .The MS media for seedlings virus-free culture are 1/4 macro-elements + 1/2 micro-elements and using edible sugar instead of sucrose,so the cost of media could be decreased to 48.7%.