创伤应激相关精神行为异常与中枢神经细胞游离钙改变

RELATIONSHIP BETWEEN ALTERATION OF INTRACELLULAR FREE CALCIUM IN CENTRAL NERVOUS SYSTEM AND TRAUMA-RELATED BEHAVIOR DISORDERS IN RATS

目的 :观察创伤应激相关精神、行为异常与中枢神经细胞游离钙改变的关系。方法 :在建立大鼠海马惊厥阈下电刺激创伤后应激障碍模型的基础上 ,采用Fura2 /AM荧光标记技术 ,动态检测了大鼠海马、额叶皮层神经细胞内游离Ca2 + 含量变化。结果 :海马神经细胞内游离钙浓度于电刺激停止后 12h明显升高 ,2 4h达高峰 ,1w时基本恢复 ;而额叶皮层只出现了短暂的细胞内钙浓度增高。结论 :海马神经细胞Ca2 + 调控异常可能是创伤应激相关精神、行为障碍的一个重要病理生理基础。

Objective: To study the relationship between trauma-related behavior disorder and intracellular free calcium alteration in central nervous system. Method: A stable and satisfied model of post-traumatic stress disorder was established and then the level of intracellular free calcium in rats' hippocampus and frontal cortex tissues was detected by Fura-2/AM. Result: It was showed that the level of intracellular free calcium in hippocampus increased significantly at 12 h after the last stimulation, and reached to the highest point at 24 h, but that in the frontal cortex increased temporally. Conclusion: It is suggested that the abnormality of calcium regulation in hippocampus after trauma plays an important role in trauma-related behavior disorder.