鼻咽癌组织中bcl-2及P^(53)蛋白表达的研究

A clinical study on bcl-2 and P^(53) proteins in the tissues of nasopharyngeal carcinoma

目的 探讨ｂｃｌ－２和Ｐ５３蛋白在鼻咽癌发生发展中的作用。方法 采用抗ｂｃｌ－２和Ｐ５３蛋白的单克隆抗体,对２５例鼻咽癌(Ｎａｓｏｐｈａｒｙｎｇｅａｌ ｃａｒｃｉｎｏｍａ,ＮＰＣ)标本和１６例正常鼻咽上皮(Ｎａｓｏｐｈａｒｙｎｇｅａｌ ｅｐｉｔｈｅｌｉａ,ＮＥ)标本进行免疫组化检测。结果ｂｃｌ－２和Ｐ５３蛋白的阳性表达率在鼻咽癌组织中分别为８４．０%(２１/２５)和７６．０%(１９/２５),与正常鼻咽上皮组相比差异有极显著性意义(Ｐ＜０．００５);鼻咽癌中有１６例同时表达ｂｃｌ－２和Ｐ５３蛋白。此外,ｂｃｌ－２蛋白多见于分化差的鼻咽癌,而Ｐ５３蛋白则在相对分化较好的鼻咽癌中较多。结论 ｂｃｌ－２原癌基因和Ｐ５３抑癌基因在鼻咽癌的发病中可能有不同的意义,二种基因的协同作用可能与鼻咽癌持续生长有关。

ve To investigate the expression of bcl-2 and P53 proteins in the tissues of nasopharyngeal carcinoma (NPC). Methods Immunohistochemical technique was used to determined the expression of bcl-2 and P53 proteins in the tissues of NPC(25 cases) and normal nasopharyngeal epithelia(NE,16 cases), and a comparison was made among them to evaluate their pathological implications in the development of NPC. Results The positive rates of bcl-2 and P53 proteins were 84. 0%(21/25) and 76. 0%(19/25) in NPC tissues respectively, significantly higher than that in NE tissues. Furthermore, the positive expression of bcl-2 was mainly seen in poorly differentiated NPC, while P53 commonly seen in more well-differentiated one. Conclusions Though the genes of bcl-2 and P53 may play different pathological roles in the developmental process of NPC, their synergy should be more important for the development of this neoplasm.