重组人表皮生长因子促人角膜缘上皮细胞生长的研究

Effect of Recombined Human Epidermal Growth Factor on Proliferation of Human Epithelial Cells

目的:探讨重组表皮生长因子(rhEGF)对人角膜缘上皮细胞生长的影响。方法:采用rhEGF作用于体外培养的人角膜缘上皮细胞,记录细胞生长曲线,并用四唑氮盐(MTT)法测定细胞增殖状况。结果:rhEGF浓度为10ng/ml时对人角膜缘上皮细胞的促增殖作用最强(P<0.05)。MTT法48h的吸光度值实验组均高于对照组(P<0.01)。结论:rhEGF对体外培养人角膜上皮细胞有明显促增生作用。

Objective:To explore the effect of recombined human epidermal growth factor (rhEGF) on proliferation of human corneal epithelial cells. Method:The rhEGF was added into cultured medium of human corneal epithelial cells. The sum of cells in different periods of corneal epithelial cells was recorded and the method of 3 [4,5 dimethylthiazol 2 yl] 2,5 diphenyltetrazolium bromide (MTT) assay was applied to estimate the state of proliferation. Results:The proliferation of human corneal epithelial cells can be promoted to the greatest extent by rhEGF at the concentration of 10 ng/ml (P< 0.05). In the experimental groups, the values of A of MTT assay at 48 h are higher than those in the control groups(P< 0.01). Conclusion:The rhEGF can promote the proliferation of human corneal epithelium.