利用cDNA微列阵技术分析胰腺癌相关基因表达谱

Analysis on gene expression profiles in pancreatic carcinoma using cDNA microarray

目的 利用cDNA微列阵技术分析胰腺癌相关基因表达谱来寻找下一步研究的目标基因。方法 利用含有 180 0 0个cDNA克隆的微列阵 ,对 2例正常胰腺组织和 4例中分化胰腺癌标本行基因表达谱分析。结果 2份混合癌组织样品分别有 14 84和 13 5 3条差异表达基因 ,表达趋势相同的有 45 5条 ,上调基因 10 2条 ,下调基因 3 5 3条 ,已知基因 2 74条 ,未知功能EST 181条 ,差异 2倍以上的基因各占 2 7.8%和 5 2 .0 % ,已知差异基因中包括抑癌基因、生长因子及其受体基因、信号传导相关基因、转录调节因子等。结论 cDNA微列阵技术为分析胰腺癌相关基因表达谱提供了有力的工具 ,MBD1、EDG1等基因和过甲基化表达调控可能在胰腺癌发病机制中起着关键作用。目的 利用cDNA微列阵技术分析胰腺癌相关基因表达谱来寻找下一步研究的目标基因。方法 利用含有 180 0 0个cDNA克隆的微列阵 ,对 2例正常胰腺组织和 4例中分化胰腺癌标本行基因表达谱分析。结果 2份混合癌组织样品分别有 14 84和 13 5 3条差异表达基因 ,表达趋势相同的有 45 5条 ,上调基因 10 2条 ,下调基因 3 5 3条 ,已知基因 2 74条 ,未知功能EST 181条 ,差异 2倍以上的基因各占 2 7.8%和 5 2 .0 % ,已知差异基因中包括抑癌基因、生长因子及其受体基因、信号?

Objective To analyze the gene expression profiles in pancreatic carcinoma by using cDNA microarray and detect target genes for further study.Methods Three mixed samples from 2 cases of normal pancreatic tissue and 4 cases of gradeⅡpancreatic carcinoma were studied by means of cDNA microarray consisting of 18?000 genes.Results There were 1?484 and 1?353 different expressed genes in two cancer samples respectively.We identified 455 genes altered with the same tendency in both samples,including 102 up regulated and 353 down regulated genes.There were 274 known genes and 181 unknown genes.27.8 % and 52.0 % genes respectively had an expression level in cancer that was 2 fold higher or lower than that in normal samples.Tumor suppressor genes,growth factor and receptor genes,signal conduction genes,transcription factor genes and so on were identified.Conclusion cDNA microarray is an efficient and high throughout method to survey gene expression profiles in pancreatic carcinoma.MBD1,EDG1 and gene hypermethylation mechanism would play an important role in the pathogenesis of pancreatic carcinoma.