神经母细胞瘤Survivin基因表达和细胞凋亡的研究

Survivin gene expression and apoptosis in neuroblastoma

目的 探讨神经母细胞瘤 (NB)Survivin表达和细胞凋亡。方法 应用免疫组织化学技术和DNA原位末端标记技术检测 2 6例NB中Survivin基因表达和细胞凋亡。结果 NB中 15例有Survivin表达阳性细胞检出 ,阳性检出率 5 7.7%。神经节母细胞瘤 (GNB)的阳性检出率为2 0 .0 % ,NB为 66.7% ;预后差组织 (UFH)组为 88.9% ,预后好 (FH)组为 41.2 % ;Ⅰ～Ⅱ及ⅣS期为 2 7.3 % ,Ⅲ～Ⅳ期为 80 .0 %。NB组、UFH组、Ⅲ～Ⅳ期标本Survivin阳性表达率高于GNB、FH、I～Ⅱ及ⅣS期标本 ,P值分别为 0 .0 82 3、0 .0 2 43、0 .0 10 5。 17例有凋亡细胞检出NB中 12例有Survivin阳性表达 ,Survivin标记指数与凋亡指数 (AI)呈负相关 (n =12 ,r =-0 .5 91)、与生存时间呈负相关 (n =12 ,r =-0 .62 9) ,AI与生存时间则呈正相关 (n =16,r =0 .5 63 )。结论 Sur vivin引起的凋亡抑制与NB发生发展关系密切 ,是患者预后的重要指标。

Objective To study Survivin gene expression and apoptosis in neuroblastoma (NB).Methods Expression of Survivin gene and apoptosis were assessed by immunohistochemistry staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling methods,respectively.Results Survivin expression was detectable in 15/26 (57.7%) cases of NB and apoptotic cells were found in 17/17 cases.A highly positive rate was found in patients with unfavourable histology (UFH),advanced staging compared to those with favourable histology (FH),early staging (P=0.024?3,0.010?5,respectively).There were negative correlation between survivin lableling index (LI) and apoptosis index (AI,n=12,r=-0.591) or survival time (n=12,r=-0.629).There was a positive correlation between AI and survival time (n=16,r=0.563).Conclusion Survivin induced apoptosis inhibition was closely related with the development of NB; To detect the level of survivin gene expression may be useful for evaluating clinical prognosis of NB.