尿激酶型纤溶酶原激活物受体和组蛋白酶D的表达与胃癌浸润和转移过程的关系

Relationship between the Expression of Urokinase-type Plasminogen Activator Receptor,Cathepsin D and the Infiltration and Metastasis of Gastric Cancer

目的 研究尿激酶型纤溶酶原激活物受体 (uPAR)和组蛋白酶D(CD)在胃癌浸润和转移过程中所起的作用。方法 采用免疫组化检测 5 9例胃癌组织中uPAR和CD的表达 ,观察它们与胃癌组织学类型、浸润深度以及淋巴结转移的关系。结果 5 9例中uPAR阳性 2 8例。在乳头状、管状腺癌和粘液腺癌中uPAR阳性率较高 ,而在低分化癌和印戒细胞癌中阳性率较低 (P <0 .0 1)。在CD中则无此差异 (P >0 .0 5 ) ;有淋巴结转移组uPAR阳性率为 6 0 .5 3% (2 3/38) ,高于无淋巴结转移组的 2 3.81% (5 /2 1) (P <0 .0 1) ,CD在淋巴结转移阳性组和阴性组阳性率分别为 79.4 9% (31/39)和 5 0 .0 0 % (10 /2 0 ) (P <0 .0 5 ) ;uPAR在早期胃癌组阳性率为 12 .5 0 % (1/8) ,侵及肌层组阳性率为 37.5 0 % (6 /16 ) ,侵犯全层组阳性率为 6 0 .0 0 % (2 1/35 ) ,差别有显著性 (P <0 .0 5 ) ,CD中则无显著差异 (P >0 .0 5 )。结论 uPAR、CD与胃癌的浸润和转移密切相关 ,uPAR与胃癌组织学类型有关。

Objective To study the effect of expression of urokinase-type plasminogen activator receptor (uPAR) and cathepsin D (CD) on the infiltration and metastasis of gastric cancer.Methods The expressions of uPAR and CD in 59 cases of gastric cancer were investigated by immunohistochemistry, SP method,and the relation of the expressions to histologic types,infiltrating depth and lymph node metastasis was also analyzed.Results Of 59 cases of gastric cancer, 28 were uPAR positive and 41 were CD positive. The uPAR positive rates in papillary,tubular and mucoid adenocarcinoma were relatively higher than in poorly differentiated carcinoma and signet-ring cell carcinoma (P<0.01), while CD had not indicated significant difference (P>0.05).uPAR positive rate in the cases with lymph node metastasis was higher than that without lymph node metastasis (P<0.01).The same result was concluded about CD (P<0.05). The difference of uPAR positive rate was significant among cases of early gastric cancer and cases with muscularis involvement and cases with whole layer infiltration. However , there was no significant difference between infiltrating depth of gastric cancer and CD.Conclusion The expression of uPAR and CD closely relate with the infiltration and metastasis of gastric cancer and uPAR relates with histologic types.