女阴尖锐湿疣及宫颈病变免疫组化与原位杂交HPV DNA的对比研究

Comparative Studies on the Expression of HPV DNA in Female Vulva Condylome Acuminata and Cervical Lesions by Immunohistochemistry and in Situ Hybridization

目的 检测非妊娠期、妊娠期尖锐湿疣、子宫颈上皮内瘤变 (CIN)、宫颈癌组织中人乳头瘤病毒 (HPVDNA)的表达 ,为临床诊治提供依据。方法 收集非妊娠期、妊娠期尖锐湿疣标本各 30例 ,宫颈上皮内瘤变 (其中CINⅠ 12例、CINⅡ 9例、CINⅢ 7例 )及宫颈癌各 2 8例 (高、中、低分化鳞癌分别为 7、16、5例 )采用免疫组化SP及原位杂交法检测HPV 6 /11DNA及HPV 16 /18DNA。结果 (1)人乳头瘤病毒感染组织不同 ,HPVDNA的阳性率有显著差异 (P <0 .0 5 ) ;(2 )原位杂交法检测优于免疫组化法。结论 (1)人乳头瘤病毒感染广泛存在于外阴尖锐湿疣、宫颈上皮内瘤变 (CIN)、宫颈癌中 ;(2 )人乳头瘤病毒与宫颈癌关系密切 ;(3)原位杂交法检测HPVDNA有助于尖锐湿疣、CIN、宫颈癌的诊断与治疗。

Objective The expression of HPV DNA in tissues of condylome acuminata(CA),cervical intraepithelial neoplasm(CIN) and cervical cancer tissues both in unpregnancy and pregnancy stages was determined with a view to lay a foundation for the clinical diagnosis and treatment.Methods The tissues of 30 biopsies of condylome acuminatum in the stage of unpregnancy and pregnancy,28 biopsies of CIN (CIN Ⅰ 12,CIN Ⅱ 9,CIN Ⅲ 7) and 28 biopsies of cervical cancer(high,middle and low differentive squamons carcinoma 7,16,5 respectively) were collected and the HPV 6/11 DNA and HPV 16/18 DNA were detected by immunohistochemistry SP and in situ hybridization,respectively.Results (1)There is a significant difference of the positive retes of HPV DNA in diffenert tissues affected with HPV ( P <0.050);(2)The method of 'in situ hybridization' is superior to immunohistochemistry.Conclusion (1)HPV infection exists extensively in vulva CA,CIN and cervical cancer tissues;(2)the pathogenesis of Cervical cancer is closely bound up with the infection of HPV;(3)The test of HPV DNA by in situ hybridization is helpful to the diagnosis and treatment of CA,CIN and cervical cancer. [