HBV-M定量检测与HBV-DNA含量水平关系的初步探讨

Preliminary Study on the Relationships between HBV Immunological Marker and HBV-DNA Concentrations

目的 研究乙型肝炎病毒标志物定量检测结果与其HBV -DNA含量的临床关系。方法 采用时间分辨免疫定量和荧光定量 -PCR技术 ,对HBV -M和HBV -DNA含量进行检测。结果 在HBsAg/HBeAg/HBcAb ,HB sAg/HBeAb/HBcAb ,HBeAg/IC及单纯HBcAb阳性的四个组别中 ,HBV -DNA阳性率分别是 97 17%、30 5 6 %、10 0 %和 2 5 % ;在HBsAg ,HBeAg ,HBeAb ,HBcAb定量检测高、低值两组HBV -DNA含量对比中 ,HBV -DNA阳性率分别为76 92 %、5 8 14 % ;10 0 %、97 2 6 % ;2 0 0 0 %、11 4 2 % ;6 4 6 2 %、2 7 6 3%。结论 HBV -DNA比HBV -M更能及时准确反映乙型肝炎病毒感染者的病程情况。

Objective To investigate the relationships between HBV infected patients immunological marker and HBV-DNA concentrations.Methods TRFIA and FQ-PCR methods were applied in the analysis of HBV immunological marker and HBV-DNA concentrations in 216 HBV infected patients.Results In patients with HBsAg+/HBeAg+/HBcAb,HBsAg+/HBeAb+/HBcAb+,HBeAg+/IC+,and HBcAb+,the HBV-DNA positive rate were 97.17%?30 56%?100%?25%.In the high and low titer groups about HBsAg+, HBeAg+, HBeAb+, and HBcAb+, the HBV-DNA positive rate were 76.92%?58 14%;100%?97 26%;20 00%?11 42%;64 62%?27 63%.Conclusion HBV-DNA is more rapid and exact than HBV immunological marker to reflect the HBV infected patients. [