低分子肝素纳米脂质体的制备及大鼠口服吸收

Study on Preparation of Low Molecular Weight Heparin Nanoliposomes and Their Oral Absorption in Rat

目的 制备低分子肝素纳米脂质体制剂 ,并进行大鼠口服吸收的研究。方法 用超声波分散法制备低分子肝素纳米脂质体 ,以正交实验设计确定最佳制备工艺 ;电镜观察其形态及粒径分布 ,以大鼠用药前后血液凝固时间的变化研究其口服吸收的促进作用。结果 低分子肝素纳米脂质体在电镜下为圆形或椭圆形 ,平均粒径为 89.6nm ,包封率为 36 .1% ;大鼠用药后血液凝固时间显著延长。

AIM The purpose is to prepare low molecular weight heparin nanoliposomes and study their oral absorption. METHODS Nanoliposomes were prepared through ultrasonication, and the shape and diameter distribution of nanoliposomes were observed under the electronic microscope. The optimal technique was chosen with orthogonal design. The oral absorption enhancing effect of nanoliposomes on low molecular weight heparin was determined by the changes of rat blood coagulation time. RESULTS Low molecular weight heparin nanoliposomes were globular or elliptic, the mean diameter was about 89.6 nm, and the encapsulated ratio was 36.1%. Rat blood coagulation time was significantly prolonged. CONCLUSION Nanoliposomes had a significant oral absorption enhancing effect on low molecular weight heparin.