大鼠心肌缺血再灌注模型中中性粒细胞与心肌损伤程度的相关性研究

The Correlation Study of Neutrophils and Myocardium Injury on Myocardium Ischemic Reperfusion in Rat

目的 观察大鼠心肌缺血再灌注模型中中性粒细胞与心肌损伤程度的相关性 ,为进一步探讨机理和寻求新药提供依据。方法 采用冠脉结扎建立大鼠心肌缺血再灌注模型 ,分别在不同时程测定血清中肌酸激酶 (CPK)、心肌组织中脂质过氧化产物 (MDA)含量 ,采用测定髓过氧化物酶 (MPO)活性来定量分析中性粒细胞。结果 随着缺血 30min后再灌注时间的增加 ,CPK、MDA和MPO都呈现时间相关性 ,而且在再灌 2h时都达到峰值。结论 在大鼠心肌缺血再灌注损伤中心肌组织的损伤程度与心肌组织中浸润的中性粒细胞有密切相关性。

AIM The purpose is to observe the correlation of neutrophils and myocardium injury on myocardium ischemic reperfusion in rat. METHODS Myocardium ischemic reperfusion model was made by occluding the left coronary artery with a snare. CPK, MDA and MPO were determined at different times. RESULTS With the increase of reperfusion time, the contents of CPK, MDA and MPO have been enhanced. After 30 min occlusion and 2 h reperfusion, the contents come to the peak value. CONCLUSON The myocardium injury correlates with the neutrophils infiltrated in the myocardium on myocardium ischemic reperfusion in rat.