自然流产绒毛中Bax、Bcl-2表达情况的研究

Study on Bax and Bcl-2 genetic expression in spontaneous abortion.

目的 研究促 /抑凋亡基因Bax/Bcl- 2在自然流产绒毛中的表达以探讨其对自然流产发生发展的作用。方法 对 4 0例早孕人工流产病例 (对照组 )和 4 0例早孕自然流产病例 (观察组 )的绒毛组织进行分析 :用免疫组织化学方法 (S -P法 )检测促 /抑凋亡基因Bax和Bcl- 2的表达。结果 对照组绒毛组织中Bax表达率为 2 0 2 1%± 3 70 % ,Bcl- 2为 2 0 91%±7 6 9% ;观察组绒毛组织中Bax表达率为 5 0 4 6 %± 11 70 % ,与对照组相比 ,差异具有高度显著性 (P <0 0 1) ,Bcl- 2为 2 3 34%± 7 78% ,与对照组相比 ,差异无显著性 (P <0 0 5 )。即 :人工流产组和自然流产组绒毛组织中都有一定量的Bax、Bcl- 2表达 ,自然流产组中Bax基因的表达增强。

To investigate Bax and Bcl-2 gengtic expression in spontaneous abortion. Methods: 40 cases of surgical aspiration and 40 cases of spontaneous abortion were examined. Bax and Bcl-2 were detected by immunohistochemical staining (S-P). Results: In surgical aspiration group each index in placenla were:Bax was 20.21%±3.70%,Bcl-2 was 20.91%±7.69%;In spontaneous abortion group, Bax was 50.46%±11.70%,it is more significant than in surgical aspiration group(P<0.01),Bcl-2 was 23.34%±7.78%,the difference has no significance than in surgical asiration group(P>0.05),That is Bax and Bcl-2 were derected quantitative in both groups, and they are more significant in spontaneous abortion group. Conclusions: Bax and Bcl-2 were both effects the development of spontaneous abortion.