组织工程方法进行特定形态骨组织构建的研究

EXPERIMENTAL RESEARCH ON NEW BONE RESTORATION IN PREDETERMINED SHAPE EMPLOYING THE METHOD OF TISSUE ENGINEERING

目的 用组织工程方法构建人下颌骨升支外形的骨组织。方法 体外培养、扩增、诱导兔骨髓基质干细胞（MSCs）,细胞长满后用基因重组人骨形成蛋白-2诱导 3天;收集细胞,按 5×107/ml的浓度混匀至 1.5％藻酸钠溶液中,然后接种于人下颌骨升支外形的多孔珊瑚中,以CaCl2固化后,植入裸鼠背部皮下组织中,于植入术后1、2个月取材,通过大体标本、X线检查、组织学检查观察新骨的形成情况。结果2月时形成的组织在裸鼠背部界限清晰,取材后观察珊瑚表面有坚硬组织形成,形状与支架材料的外形基本一致,X线检查证实珊瑚已有明显吸收,在其表面有明显X线组射影形成;组织学检查表明,在珊瑚表面及内部的孔洞内均有大量新骨形成,新骨形成过程为软骨内成骨。结论 用组织工程方法可以构建出特定形态骨组织,因此适于进行颌面部骨缺损的修复。

ve To fabricate bone grafts in the predetermined shape of human mandibular rumas employing the method of tissue engineering. Methods Marrow stem cells (MSCs) were cultured, expanded and induced in vitro. Cells were mixed in 1.5% alginate acid sodium at the density of 5 × 107/ml and then seeded into porous natural coral (NC) in the shape of human mandibular rumas in 1 .0ml cell suspension. After solidification by 3% Cacl2 for 5min, four cell-coral complexes were implanted subcautaniously into nude mice back. Two months after operation, bone formation was observed by gross inspection, X-ray examination, scanning electronic microscope observation and histologic observation. Results New bone grafts in the shape of human mandibular rumas were successfully restored two months after implantation, and maintained the initial shape of the NC scaffolds (4 of 4). X-ray examination showed large amount of X-ray blocking shadow formation. NC was partially absorbed. New bone formation could be observed by electronic microscope observation and histological observation on the surface and in the pores of NC. Conclusion It is an effective method to fabricate bone graft in specific shape by seeding osteogenesis cells into natural coral of specific shape.