用米诺环素处理病变牙骨质的体外效果

The effect of minocycline-HCl on diseased cementum in vitro

目的 用盐酸米诺环素处理牙周炎患牙的牙骨质 ,观察其表面结构和牙周膜细胞在其上附着的影响。方法 选暴露于牙周袋的根面 ,制备成牙骨质片 ,实验组用 2 .5 %盐酸米诺环素液涂擦 ,对照组用生理盐水。用扫描电镜观察不同处理后 ,根面牙骨质的超微结构及牙周膜细胞在不同处理根面上附着生长的情况。结果 盐酸米诺环素处理后的根面 ,无玷污层 ,有胶原纤维暴露 ;牙周膜细胞在其上的伸展和生长优于对照组。结论 盐酸米诺环素处理可使病变牙骨质胶原纤维暴露 。

Objective To investigate the effects of minocycline-HCl on diseased cementum and on the attachment of human periodontal ligament (HPDL) cells to the conditioned root surfaces.Methods Cementum chips were obtained from roots exposed to periodontal pockets. After root planing, the chips were burnished with minocycline-HCl , and with saline burnishing as control. The root slices were divided into four groups, tow of those were placed in culture dishes with human periodontal ligament cells incubated for 24 hours. And then the media was removed and samples were fixed and processed for scanning electron microscopy (SEM). The surface morphology of cementum chips and HPDL cells were observed using SEM.Results Diseased root surfaces that were root planed revealed a flat, smooth surface, without smear layer. Mimocycline-HCl conditioned specimen showed a flat surface with numerous fiber-like structures, on which there was no smear layer. On Minocycline conditioned cementum surfaces, layered HPDL cells covered entirely the root chip surfaces at 24 hours. HPDL cells were flat, elongated with interconnected cytoplastmic extensions. The spreading of the cells make the contours of the cells hardly visible. On root planed alone surfaces, monolayer HPDL cells covered the root chips only partly at 24 hours. HPDL cells were flat with less spreading extensions. Cells were not as strong as that on minocycline treated root surface.Conclusion In view of morphology, minocycline-HCl conditioning causes demineralization of diseased cementum and exposes the collagen fibrils, which promote the attachment and growth of HPDL cells on the diseased root surface.