P物质、神经肽Y和生长抑素在大鼠舌下腺的免疫组织化学观察

Studying Immunohistochemically the Existance and Distribution of Substance P,Neuropeptide Y and Somatostatin in Rat's Sublingual Gland

目的 :观察P物质 (SP)、神经肽Y(NPY)和生长抑素 (SOM)在大鼠舌下腺的分布。方法 :用免疫组织化学ABC法。结果 :大鼠舌下腺纹状管上皮细胞分别呈SP、NPY和SOM免疫反应阳性 ,腺细胞为阴性。结论 :大鼠舌下腺纹状管上皮细胞内含有多种神经肽 。

Objective: Substance P. neuropeptide Y and somatostatin belong totally to neurotransmitter, they elicit very important role in regulating neuroendocrine activities of whole body. The purpose of this study was to observe the existence and distribution of these neurotransmitters in rat sublingual gland. Methods: Wax-embedded specimen preparation and immunohistochemical ABC method were done routinely. All antisera were purchased from Sigma and INC Company USA. Results: The striated ducts of epithelial cell in rat sublingual gland showed SP, NPY and somatostatin immunoreaction positive,but the acinar cells negative. Conclusion: In the gland epithelial cells, there exist three neuropeptides which might directly participate in the regulation of secretory activity and local blood supply or indirectly exert control over the regulation through the involvement of intermedia such as hormone.