月见草花浸液对鼠肠道平滑肌收缩活动的影响

Effect of Water-soluble Extractives From Oenothera Flower on the Contractilities of Small Intestines of Guinea-pig and Mouse

目的 :观察月见草花浸泡液对豚鼠离体回肠及小白鼠在体小肠收缩活动的影响。方法 :应用离体豚鼠回肠实验法及小白鼠小肠推进运动实验法观察不同剂量的月见草花浸泡液对鼠肠道平滑肌的作用。结果 :应用不同剂量 ( 1 7mg/ml,3 3mg/ml,5 0mg/ml)月见草花浸泡液后 ,豚鼠离体回肠收缩幅度持续升高 ,与未用药前相比较有极显著性差异 ( P <0 0 0 1 ) ;小白鼠在体小肠的收缩活动随着浓度的增加也有显著性加强作用 ,与生理盐水组相比较 ,灌服 1 0 %和 2 0 %月见草花浸泡液后均有极显著性差异 (P <0 0 1 )。结论 :月见草花浸泡液对鼠离体及在体肠道平滑肌收缩活动均有明显加强作用 。

Objective: To observe the effect of water-soluble extractives from oenothera flower on the contractilities of isolated ileum from guinea pig and small intestine which was pulled out but still connected in situ to the mouse just died by experimental violence. Methods: Contractive amplitude and propulsive force of experimental murine intestines were used as indicators of measuring intestinal contractivities before and after being given different doses of water-soluble extractives of oenothera flower. Results: After giving different doses of oenothera's flower water-soluble extractives (1.7mg/ml, 3.3mg/ml, 5.0mg/ml), the contraction amplitude of isolated ileum of guinea pigs continuously went up and showed a statistically significant difference, compared with that before loading. The contractions of mouse small intestines strengthened along with the doses increase, after being given 10% and 20% extractivesm the difference was statistically significant compared with that receiving N.S. only.Conclusion: Water-soluble extractives of oneothrera flower reveals an obvious effect on the contractilities of isolated ileum of guimea pig and small intestine of mouse and it also shows certain dose- effect relationship.