RNA编辑酶ADAR1对淋巴细胞系增殖的影响

Influence of RNA editase ADAR1 on mouse lymphocyte proliferation

目的 探索ADAR1活性升高对淋巴细胞细胞增殖的影响。方法 ①构建反转录病毒载体 :PCR从小鼠cDNA中放大ADAR1全长基因 ,连入反转录病毒MIEV载体 ,感染包装细胞后分泌病毒颗粒 ;②病毒颗粒感染T淋巴细胞CTLL2和B淋巴细胞A2 0 ,观察细胞增殖的变化 ;③MTT法测定细胞增殖率。结果 ADAR1/MIEV病毒感染淋巴细胞后 ,使细胞增殖速度减慢。结论 ADAR1在淋巴细胞发挥功能方面起有重要的作用 ,但哪些物质需要编辑以及具体机制如何 ,需要进一步研究。

Objective To investigate the influence of ADAR1 on lymphocyte proliferation.Methods ①ADAR1 gene was amplified using PCR from mouse cDNA and ligated into retrovirus vector, MIEV. The plasmid was transformed into package cell line and ADAR1/MIEV virus was produced; ②T lymphocyte, CTLL 2, and B lymphocyte, A20 were infected with ADAR1/MIEV; ③ The cell proliferation, infected with MIEV virus, was measured using MTT method.Results The proliferation of A20 and CTLL 2 cells were reduced when ADAR1 activity in those cells was increased.Conclusions ADAR1 might play an important potential role in the lymphocyte function. The ADAR1 downstream gene and detail mechanism working in lymphocyte should be further investigated.