镁对内皮细胞氧化还原相关基因表达的影响

EFFECT OF MAGNESIUM SUPPLEMENTATION ON REDOX- REGULATION RELATED GENES EXPRESSION IN ENDOTHELIAL CELLS

目的 : 研究 Mg2 + 对人脐静脉内皮细胞中氧化还原相关基因 c- fos、c- jun、p5 3和ref- 1表达的影响。方法 : 免疫组织化学染色 ,并用图象分析系统对染色切片进行半定量分析。结果 : 与正常对照组相比 ,H2 O2 组 c- fos、c- jun、p5 3和 ref- 1的表达增强 ,具有显著性差异 ( P<0 .0 5 ) ;Mg2 + 各剂量组 ref- 1表达增强 ( P<0 .0 1 ) ;与 H2 O2 组相比 ,H2 O2 + Mg2 + 各剂量组 c- fos、c-jun和 p5 3表达减弱 ,ref- 1表达增强 ( P<0 .0 1 ) ;H2 O2 + 0 .3mmol/L Mg2 +组 p5 3表达强于 0 .3mmol/L Mg2 + 组 ,具有显著性差异 ( P<0 .0 1 ) ,H2 O2 + 0 .3mmol/L Mg2 + 、H2 O2 + 0 .6mmol/LMg2 +组和 H2 O2 + 1 .2 mmol/L Mg2 +组 ref- 1表达强于相应的 Mg2 +剂量组 ,具有显著性差异 ( P<0 .0 5 ,P<0 .0 1 )。结论 : Mg2 + 通过影响与细胞氧化还原状态有关的 c- fos、c- jun、p5 3和 ref- 1基因表达来影响氧化还原信号转导通路 ,可能与其抗氧化作用有关。

Objective: To study the effect of Mg 2+ onc fos, c jun, p53 and ref 1, that are transcription factors related to redox regulation, in cultured human umbilical vein endothelial cell. Methods: Imunohistochemistry techniques and image analysis system were used. Results:In the group of H 2O 2, the expresson of c fos, c jun, p53 and ref 1 was increased compared to the normal control group (P<0.05). The expression of ref 1 in the group of Mg 2+ at all doses were stronger than that of normal control group (P<0.01). When compared with the group of H 2O 2, the expression of c fos, c jun, and p53 in the group of H 2O 2+Mg 2+ at all doses was lowered significantly (P<0.01), but the expression of ref 1 increased (P<0.01). Conclusion: It suggests that the antioxidant effect of magnesium supplementation is probably related to its action on the expression of redox regulation related genes c fos, c jun, p53 and ref 1 and subsequent redox signal transduction.