莲房原花青素对氧自由基和脂质过氧化的作用

EFFECTS OF PROCYANIDINS EXTRACT FROM THE LOTUS SEEDPOD ON REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION

目的 : 观察莲房原花青素 ( LSPC)对活性氧自由基 O·-2 、· OH和肝脂质过氧化损伤的作用。方法 : ( 1 )应用化学发光法检测 LSPC对 O·-2 和· OH的清除作用 ;( 2 )体外大鼠红细胞、肝线粒体温育引起肝脂质过氧化物 ( LPO)含量变化 ;( 3)体外大鼠肝匀浆温育 ,Fe2 +、H2 O2 诱导引起 LPO含量变化 ;( 4 )小鼠连续口服不同剂量 LSPC 7d后 ,一次性灌胃 5 % CCl4油溶液 1 0 ml/kg bw,2 4 h后检测肝和血浆中超氧化物歧化酶 ( SOD)活性、谷胱甘肽 S-转移酶 ( GST)活性以及脂质过氧化物 ( LPO)含量。结果 : LSPC能有效地清除活性氧自由基 O·-2 和· OH,其 IC50 值分别为 1 69.0 mg/L和 1 0 5 .3mg/L;不同剂量 LSPC能明显抑制体外大鼠红细胞、肝线粒体、肝组织LPO产生 ;口服中、低剂量 LSPC能显著降低 CCl4中毒小鼠体内 LPO含量 ,提高 SOD、GST活性。结论 : 适量 LSPC具有抗活性氧自由基和脂质过氧化的作用 ,可能具有护肝功能。

Objective: To study the effects of procyanidins extract from the lotus seedpod (LSPC) on active oxygen radicals and lipid peroxidation. Methods: (1) The scavenging effects of LSPC on O ·- 2 and ·OH was investigated by using chemiluminescence method; (2) The effects of different concentrations of LSPC on RBC auto oxidation and LPO formation of rat liver mitochondria in vitro were observed; (3) The LPO level of liver homogenates incubated with Fe 2+ and H 2O 2 in vitro were determined; (4) the mice were treated with a single intragastric feeding of 5% CCl 4 soya bean oil solution (10ml/kg bw) after seven days supplementation of LSPC 100, 200, 400 mg/(kg bw·d). The activities of SOD, GST and the level of LPO in liver and plasma of CCl 4 toxic mice were determined. Results: In vitro, LSPC 169.0mg/L or 105.3mg/L was shown to markedly scavenge O ·- 2 produced in xanthine/xanthine oxidase and ·OH in Fenton systems respectively, and could significantly inhibit the formation of liver LPO. In vivo, LSPC (100,200mg/kg) evidently reduced the level of LPO and increase the activities of SOD and GST. Conclusion: It suggests that LSPC may be used as an antioxidant and might effectively protect liver from the injury due to lipid peroxidation.