维生素E对微血管生成的抑制作用

EFFECT OF VITAMIN E ON ANGIOGENESIS

目的 : 探讨三种浓度维生素 E( VE)对微血管生成的作用。方法 : 采用无血清培养基 ,通过胶原凝胶立体培养大鼠主动脉环共 2 8d,并计数微血管绘制生长曲线 ,采用免疫组织化学技术检测凝血第八因子相关抗原 ( FVIII- RAg)在新生血管中的表达 ,确定其为新生内皮细胞的性质 ;用酶联免疫测定法检测凝血第八因子 ( FVIII)在培养液中的浓度变化确定内皮生长程度。结果 : 0 .2 g/ L VE和 0 .1 g/ L VE均具有明显抑制血管生成作用 ( P<0 .0 1 ) ;0 .0 5 g/ L VE对血管生成无影响 ( P>0 .0 5 )。 VE的抑制效果主要作用在血管生成期和衰退期 ( 1 0～ 2 5 d)。结论 : 1 .无血清立体培养微血管可用于研究液相和固相血管生成激动剂与抑制剂的作用。 2 . 0 .1 g/ L VE和 0 .2 g/ L VE均具有抑制血管生成作用。

Objective: To investigate the effects of different concentrations of vitamin E (VE) on angiogenesis and its possible mechanism. Methods: The rings of rat aorta were embedded in gels of collagen and cultured in a serum free medium for 28 days. Curves of microvessels growth were generated by counting the number of newly formed microvessels every day with an inverted microscope. Photos were taken at the same time. Expression of factor FVIII related antigen (FVIII RAg) in newly formed microvessels was detected by immunohistochemistry. Concentration change of FVIII R in medium was evaluated by ELISA. Results: Both 0.2 g/L and 0.1 g/L VE showed significant inhibitory effect on angiogenesis (P<0.01), while 0.05 g/L VE no inhibitory effect (P>0.05). The inhibitory effect of VE was expressed in the phases of microvessel growth and decline (10-25 d). Conclusion: 1.Three dimensional collagen gels culture of microvessel in serum free medium can be used as a sensitive assay for study of soluble and solid phase angiogenic agonists and antagonists. 2. Both 0.1 g/L and 0.2 g/L VE inhibit angiogenesis, and VE has no toxicity and no drug resistance.