对2-(2、3、5—三氮唑偶氮)—5-二甲氨基苯甲酸的研究

Research on the Compound of Nucleic Acids and Cu(Ⅱ) - 2-(2-3-5 -triazolyazo) - 5-dimethyminobenoic Acid

研究了新显色剂2-(2、3、5-三氮唑偶氮)-5-二甲氨基苯甲酸(简称TZADMAB)与Cu(Ⅱ)的显色反应.在一定浓度的KNO3介质中,pH=2.5,室温条件下,与Cu(Ⅱ)形成蓝紫色配合物,其组成比为Cu(Ⅱ):TZADMAB=1:2,稳定常数K=3.4×1013,最大吸收波长为550nm.在此条件下,Cu(Ⅱ)-TZADMAB配合物与脱氧核糖核酸(DNA)能迅速生成超分子配合物,使配合物的吸光度迅速降低,因此利用Cu(Ⅱ)-TZADMAB配合物吸光度降低的原理可以检测脱氧核糖核酸.脱氧核糖核酸质量浓度在0.02～0.12mg/ml范围内服从朗伯-比尔定律.

A study of the developing reaction of a new developer 2- (2-3-5 - triazolyazo) - 5 - dimethylaminobenzoicacid (TZADMAB) and Cu(Ⅱ) was made. In a proper KNO3 and a pH of 2.5, it forms a blue-and-violate compound atroom temperature. The mole ratio of this compound is Cu(Ⅱ): TZADMAB =1:2, and its stabilization constant equals3.4× 1013, the maximum absorption, 550 nm. Under such circumstances, Cu(Ⅱ) - TZADMAB compound and DNAcan rapidly form a super molecular compound, which soon reduces the compound's ability to absorb light. Therefore,DNA can be determined from the reduction of light-absorption ability of Cu(Ⅱ) - TZADMAB compound, as theconcentration of DNA ranging from 0.02 ~0.12 mg/ml is subject to Lamber - Bill Law.