重组大肠杆菌高效表达hbFGF的培养条件

Study on the Cultural Conditions of High Expression of Human Basic Fibroblast Growth Factor by Recombinant Escherichia coli hbFGF/BL21(DE3)pLysS

对工程菌hbFGF/BL2 1(DE3) pLysS高效表达hbFGF的培养条件的研究结果表明 ,接种量 (φ)为 5 % ,当培养液菌体浓度为 0 .8OD6 0 0 时 ,添加 0 .5mmol/L诱导剂IPTG可以获得较高的hbFGF表达量 ,30 %的溶解氧和较低的醋酸浓度有利于hbFGF的表达 .

The results of the study on the cultural conditions of high expression of hbFGF by recombinant Escherichia coli hbFGF/BL21(DE3)pLysS show that with inoculum quantity as 5% of the cultural medium, adding 0.5 mmol/L IPTG as revulsant to the leavening when its OD 600 reaches 0.8, higher expre_ ssion of hbFGF can be gained. 30% dissolved oxygen and lower concentration of acetate acid are conductive to hbFGF expression.