高效毛细管电泳法测定复方氯霉素洗剂中醋酸曲安奈德和氯霉素的含量

Determination of Triamcinolone acetonide acetate and Chloramphenicol in Compound Chloramphenicol Liniment by HPCE

目的 :建立同时测定复方氯霉素洗剂中醋酸曲安奈德和氯霉素的方法。方法 :采用高效毛细管电泳法 ,以10 0 mmol/ L 硼砂溶液 (用稀磷酸溶液调节至 p H7.0 )为运行缓冲液。运行缓冲液的溶媒为水 -甲醇 (7∶ 3)。稀释样品的缓冲液为 0 .5 %硼砂的醇 (7∶ 3)溶液。分离电压为 2 3k V ;检测波长为 2 5 4 nm ;工作温度为 2 5℃ ;高气压进样 4秒。测定氯霉素时以头孢拉定作为内标物 ,测定醋酸曲安奈德时以已知含量的氯霉素作为内标物。未涂渍毛细管 :4 7cm×75 μm i.d.,有效长度为 4 0 cm。结果 :平均回收率 :醋酸曲安奈德为 99.4 % ,RSD 1.2 % (n =6 ) ;氯霉素为 99.7% ,RSD 1.5 % (n =6 )。结论 :本法分离效率高 ,结果比较准确 ,与高效液相色谱法相比结果无显著性差异。为同时测定醋酸曲安奈德和氯霉素含量的又一种方法。

Objective: To establish a method for the determination of both triamcinolone acetonide and chloramphenicol in Compound chloramphenicol liniment by HPCE. Method: A running buffer consisting of 100 mmol/L sodium tetraborate was prepared and adjusted to pH 7.0 with diluted phosphoric acid. The running buffer was prepared by mixing water and methanol in 7∶3 (v/v). The dilution liniment was prepared by mixing water and methanol in 7∶3 (v/v), containing sodium tetraborate at 0.5%(w/v). Electrophoretic separations were conducted at 23 kV voltage. A fused silica capillary which is 47 cm×75 μm i.d., and 40cm available length was inserted in a capillary cartridge, thermostated to 25 ℃. Samples were injected for 4s. The analysis were monitored at 254 nm. Cefradine was used as internal standard when chloramphenicol was determined and determined a chloramphenicol was used as internal standard when triamcinolone acetonide acetate was determined. In addition, the paper compared HPCE with HPLC. Results: The average recovery: triamcinolone acetonide acetate was 99.4%, RSD=1.2%(n=6); Chloramphenicol was 99.7%, RSD=1.5%(n=6).Conclusion: The method is a highly efficient and selective separation method. The results of HPCE and HPLC is no remarkable difference.