高效液相色谱法测定肌酸中肌酐含量

High Performance Liquid Chromatographic Analysis of creatinine in creatine

目的 :为快速准确测定肌酐建立 HPL C分析方法。方法 :C1 8柱 (4.6 m m× 2 5 0 mm,10 μm)。流动相 :0 .0 1mol/ L磷酸钾 (磷酸调 p H至 6 .0 )。检测波长 :2 35 nm。结果 :肌酐在 0 .12～ 0 .6μg/ ml浓度范围内 ,呈良好的线性关系 ,回归方程计算结果为 Y =1.46 1e- 5X +0 ,r =0 .9992 。RSD=1.75 % (n=5 )。 3批样品肌酐含量分别为175× 10 - 6 、35 2× 10 - 6 、2 6 7× 10 - 6 ,样品重现性试验 RSD =1.14% (n =5 )。结论 :应用本方法可快速、简便、准确测定肌酸中杂质——肌酐的含量。

Objective: To develop a HPLC method for assaying Creatinine; Method: C 18 column (4.6 mm×250 mm, 10 μm).methanol : 0.01 mol/L potassium phosphate solution pH6.0 as mobile phase. Detector at 235 nm. Results: It has a good linear correlation in 0.12～0.6 μg/ml, Y=1.461e -5 X+0, r=0.999. RSD=1.75%(n=5). The content of creatinine in the creatine sample were as follow: l75×10 -6 , 352×10 -6 , 267×10 -6 , RSD=1.14%(n=5); Conclusion: This method provided simple and rapid method to analyze creatinine in creatine.