摘要
目的 :建立成方制剂清热凉解糖浆中有效成分绿原酸含量测定的方法。方法 :采用高效液相法 ,以岛津Shim- pack VP- ODS柱为固定相 ,流动相为乙腈 - 0 .3%冰醋酸 (10∶ 90 ) ,流速 1.0 ml/ m in,检测波长为 32 4nm。结果 :绿原酸在 0 .0 5 0 0 4～ 0 .6 2 5 5μg范围内具有良好线性关系 ,r =0 .9999,平均回收率 99.6 % ,RSD =1.8% (n =6 )。结论 :该方法简便、准确 ,适合该制剂中绿原酸的含量测定。
Objective: To establish a HPLC method for the determination of Chlorogenic acid in Qingreliangjie Syrups. Method: Using Shim pack VP ODS column, 0.3% glacial acetic acid acetonitrile(90∶10) as mobile phase, flow rate is 1.0ml/min, absorbance determination was set at 324 nm. Results: Chlorogenic acid in Qingreliangjie Syrups was baseline separated and determined. The linearity ranges were 0.050 04～0.625 5 μg , correlation coefficient was 0.999 9. The average recovery was 99.6%(RSD=1.8%, n=6). Conclusion: This method is simple, accurate, it can be used in the quality control of Qingreliangjie Syrups.
出处
《天津药学》
2002年第3期77-78,共2页
Tianjin Pharmacy
