摘要
克隆了Cre基因 ,分别构建了真核表达载体 pEF EBFP Cre和 pGEX Cre,在大肠杆菌中表达了Cre重组酶 ,并证明了重组酶的删除特性 。
Cre/loxP system is the powerful tool for the study of gene expression and control. We construct the eukaryotic expression vector pEF EBFP Cre and prokaryotic expression vector pGEX Cre. The Cre recombinase is expressed in E. coli and its deletion property is observed. The expressed Cre recobinase could be used in the further transgenic mammary bioreactor research.
出处
《高技术通讯》
EI
CAS
CSCD
2002年第6期31-33,共3页
Chinese High Technology Letters
基金
中国科学院知识创新工程 (KSCX1 0 5 0 1)项目资助
