牙鲆(Paralichthys olivaceus)淋巴囊肿病毒的分离

Diagnosis of Lymphocystis Disease of Cultured Japanese Flounder Paralichthys olivaceus

通过不同的样品处理和离心方法 ,发现了影响淋巴囊肿病毒 (LCDV)得率和纯度的因子 ,通过消除该因子并结合计算机软件设计程序 ,建立了高纯度LCDV的快速分离技术。

A series of specific primers are designed based on the major capsid protein(MCP) of lymphosystis disease virus (LCDVcn) in Paralichthys olivaceus, the conservative sequence part of the major capsid protein (MCP) is chosen as target gene. 172bp virus DNA fragment can be expressed from the primers, and the minimum detect limitation is 0.0183ng. LCDV have been detected by PCR method from the blood, gill, liver, spleen, intestines and stomach of n Paralichthys olivaceus which have been artificially infected for 3days, and from the of spontaneously iParalichthys olivaceus. Our results show that PCR method is effective in early detecting of LCDV.