摘要
采用不连续聚丙烯酰胺凝胶电泳技术 ,研究了中国栉孔扇贝和日本栉孔扇贝的两组杂交子代群体的 1 4个位点 2 4个等位基因。将它们各自的自交子一代作为亲本的参照组 ,对这四组子代群体进行了遗传变异分析。结果表明 :四组群体具有相同的基因位点控制同工酶的表达 ,但各位点等位基因的频率有所不同。统计结果表明 :杂交群体的遗传变异明显高于自交群体的遗传变异。四组群体的多态位点比例 (P 99)分别为 3 6 3 6%(CP中自交组)、3 0 77% (JP日自交组 )、3 0 77% (JFCM日雌中雄杂交组 )、3 0 77% (JM CF日雄中雌杂交组 ) ,平均杂合度观测值 (Ho)分别为 0 1 2 73 (CP)、0 1 0 5 5 (JP)、0 1 5 3 6(JFCM)、0 1 72 7(JMCF) ,各位点平均有效等位基因数 (Ae)分别为 0 73 1 8(CP)、1 671 8(JP)、1 695 5 (JFCM)、1 692 7(JMCF)。杂交优势已初步显示出来。
Polyacrylamide gel electrophoresis(PAGE) was used to determine the genetic variation of two populations of self bred progeny of Chlamys farreri from China(CP) and Japan(JP) and their reciprocal hybrids using 9 isozymes 14 loci?The results show that the genetic diversity of filial generations is higher than self bred progeny's,the hybrid vigor has manifested at metabolization level?The proportion of polymorphic locus (P.99) in F1 generations was 31%～36.36%, the mean observed heterozygosity (Ho) of JMCF(Japanese male×Chinese female)was the highest with 0.1727, the rest populations were: JFCM(Japanese female×Chinese male) 0.1536?CP 0.1273 and JP 0.1055,the effective number of allels per locus in F1 generations:CP's was the highest with 1.7318 ,JP's was the lowest with 1.6718,the filial generations'values were in the middle of the CP's and JP's and between them there was no brightly difference. In general, the hybridizition technique between subspecies for improvement of quantitative and qualitative traits is practicable.
出处
《高技术通讯》
EI
CAS
CSCD
2002年第6期101-105,100,共6页
Chinese High Technology Letters
基金
973计划(G19990 12 0 0 8)资助项目
