血管紧张素Ⅱ刺激肝星状细胞核酸、蛋白质及胶原合成

Angiotensin Ⅱ stimulates the synthesis of nucleic acid, protein and collagen in cultured hepatic stellate cells in rats

目的 研究不同浓度血管紧张素Ⅱ (AngⅡ )对大鼠肝星状细胞 (HSC)增殖和胶原合成的影响。方法 采用酶法分离大鼠肝星状细胞 (HSC) ,应用3 H TdR(胸腺嘧啶核苷 )、3 H Leu(亮氨酸 )、3 H Pro(脯氨酸 )掺入检测 10 -9～ 10 -5mol/L不同浓度AngⅡ对HSC的DNA、蛋白质及脯氨酸合成的影响。结果 10 -9～ 10 -6mol/LAngⅡ能促进HSC对3 H TdR的掺入率 (P <0 .0 5 ) ,但只有 10 -6mol/L和10 -7mol/L浓度的AngⅡ才能显著增加HSC对3 H Leu的掺入率 (P <0 .0 1) ;而 10 -9～ 10 -6mol/LAngⅡ均能促进HSC对3 H Pro的掺入率 (P <0 .0 5 ) ,且呈剂量依赖关系。

Objective To investigate the effects of different doses of angiotensin Ⅱ(Ang Ⅱ) on hepa tic stellate cells(HSC) proliferation and collagen synthesis in rats. Methods The pronase E and collagen Ⅰ were used to isolate HSC, 3H TdR, 3H Leu and 3H Pro incorporation methods were used to evaluate the effects of different doses of AngⅡ on HSC DNA, protein and proline synthesis. Results It showed that 10 -9 ～10 -6 mol/L AngⅡ could significantly increase the 3H TdR incorporation rate of HSC ( P <0.05), but only 10 -6 mol/L and 10 -7 mol/L AngⅡ could significantly increase the 3H Leu incorporation rate of HSC ( P < 0.01); 10 -9 ～10 -6 mol/L AngⅡ could significantly increase the 3H Pro incorporation rate of HSC in a dose dependent style. Conclusions The appropriate dose of AngⅡ may promote HSC in proliferation and collagen synthesis.