抗体-免疫印迹方法检测肝癌甲胎蛋白异质体

Detection of Alpha-fetoprotein Alloplasm of Hepatocellular Carcinoma by Antibody-immunoblotting

目的:建立一种随访肝硬化患者早期发现肝细胞癌（HCC）的方法。方法:用生物素抗体亲和印迹方法检测慢性肝病患者血清甲胎蛋白异质体AFP-L3和AFP-P4+P5。结果:HCC患者AFP-L3和AFP-P4+P5水平分别为（49.57±18.09）％和（43.96±14.03）％,明显高于慢性肝病患者的（4.98±5.53）％和（10.0±11.0）％。其诊断肝癌敏感性分别为96.55％和93.75％,特异性为100％和88.24％。结论:该方法敏感性高,特异性强,更方便临床随访高发人群,早期发现HCC。

Objective: To establish a method of detecting early hepatocellular carcinoma (HCC) during periodic follow-up of patients with liver cirrhosis. Methods: The level of alpha-fetoprotein alloplasm (AFP-L3 and AFP -P4+ P5) was measured by antibody-immunoblotting in 75 patients with chronic liver disease. Results: The levels of AFP-L3 and AFP - P4 + P5 in patients with HCC[(49.57±18.09)% and (43.96±14.03)%] were significantly higher than those in patients with non-HCC liver disease[ (4.98±5.53)% and( 10.00±11.00)% ] (P <0.05 and P<0.01) .The sensitivities of AFP-L3 and AFP - P4+P5 were 96.55% and 93.75% respectively. Their specificities were 100.00% and 88.24%) respectively. Conclusion : The measurement is of higher sensitivity, specificity and convenient in the early recognition of HCC.