γ射线照射对两种组织修复细胞的直接效应与W_(11)-a_(12)的作用

Effects of γ irradiation on rate of wound healing in a scored confluent monolayer of cells and the repair-promoting role of W_(11)-a_(12)

目的 探讨电离辐射是否对成纤维细胞和血管内皮细胞的增殖游走功能具有直接抑制作用及其抑制程度 ,以及W1 1 a1 2 的促愈作用。方法 采用培养的单层 3T3细胞 (小鼠胚胎FBs株 )和培养的单层ECV30 4细胞 (人脐静脉内皮细胞株 )划痕伤口模型进行离体照射 ,观察痕隙闭合速度。结果 在 6Gy照射后培养的单层 3T3细胞与单层L ECV30 4细胞划痕“伤口”闭合明显延缓。单层 3T3细胞在伤后 10h对照组伤口完全闭合 ,而照射组仅闭合 77% ;照射组单层ECV30 4细胞在伤后 12h仅为对照组的 83 6 %。W1 1 a1 2 用药组对单层 3T3细胞与ECV30 4细胞“划痕”伤口的闭合均有促进作用。结论 放射损伤对成纤维细胞与血管内皮细胞的增殖游走有直接抑制作用 ,这是伤口难愈的重要原因之一。促进伤口细胞游走和增殖是W1 1 a1 2 作用的一个重要途径。

Objective\ To investigate the effects of ionizing radiation on healing rate of experimental wound in a scored confluent monolayer of fibroblasts and vascular endothelial cells and the repair promoting effect of W\-\{11\} a\-\{12\}. Methods\ The healing rates of the experimental wound in a scored confluent monolayer of 3T3 cells or ECV304 cells irradiated with 6 Gy 60 Co gamma rays were assayed by measuring the width of the wound. Results\ After irradiation,the closure of scored wounds both in a confluent monolayer of 3T3 cells and in that of ECV304 cells was significantly delayed.The scored wound in a confluent monolayer of 3T3 cells was completely closed in the sham irradiation group,but it was only 77% in the irradiation group at the tenth hour post wounding.The healing rate of the scored wound in a confluent monolayer of irradiated ECV304 cells was 83 6% of that in the sham irradiation group W\-\{11\} a\-\{12\} had good promoting action on the closure of wounds in scored confluent monolayers of these two kinds of cells. Conclusion\ The direct inhibitory effects of irradiation on the proliferating and migrating capacity of both fibroblasts and vascular endothelial cells might be one of the important reasons for the delay of healing in irradiation impaired wounds and W\-\{11\} a\-\{12\} could promote healing of irradiation impaired wound by means of enhancing cell migratation and proliferation directly.\;