不同剂量软X射线照射对大鼠伤口愈合影响规律的研究

Regularity of wound healing in rats irradiated locally with different doses of soft X-rays

目的 研究软X射线局部照射对创伤愈合的影响规律。方法 采用大体观察、组织病理学、免疫组化 (胶原染色 )和图像分析等方法 ,动态观察不同剂量软X线局部照射后大鼠伤口愈合的过程。结果 0 5 0、1 0 1、1 96、3 2 6、4 0 0、5 2 1Gy组照射侧的创面愈合时间比对照侧分别延长了 1 6、4 2、5 4、6 6、8 2、9 4d ,7 0及 10 0Gy组则创面不愈 (>40d)。各组的已愈创面百分率在 3～ 9d之间的变化幅度均比对照侧小 ,随着照射剂量的增大 ,变化的幅度亦减小 ,表明创面愈合速度减慢。照射后伤口组织胶原的合成降低 ,排列紊乱 ,肉芽组织的结构不规则。结论 局部软X线照射后 ,大鼠伤口愈合延迟 ,在 0 5 0～ 5 2 1Gy之间延迟时间随照射剂量的增大而延长。

Objective\ To study the regular patter of wound healing in rats irradiated locally with different doses of soft X rays. Methods\ Rats were locally irradiated,and wounded immediately thereafter.Gross observation,histopathology and immunohistochemistry examinations,and image analysis were used to study the wound healing process. Results\ We found that the delayed time of wound healing induced by soft X ray irradiation of 0 50,1 01,1 96,3 26,4 00,5 21 Gy was 1 6,4 2,5 4,6 6,8 2 and 9 4 days,respectively. Irradiation with 7\^0 and 10\^0 Gy caused failare of wound healing (up to 40 days).Compared to the non irradiated wounds,the healing rates of irradiation impaired wounds were lower during the whole healing process.From day 3 to day 9 after irradiation,the healing rates decreased along with increase of the radiation dose,indicating the key phase of wound healing was delayed.After irradiation,the collagen synthesis was decreased,its arrangement was disordered,and the structure of granulation tissue was irregular. Conclusion\ Soft X rays irradiation may cause a delay of wound healing in a dose dependent manner,and irradiation with 7\^0 and 10\^0 Gy causes failure of wound healing.\;