小鼠巨噬细胞数量与形态及其IL-12基因表达放烧复合效应的实验研究

Effect of combined radiation-burn injury on cell number,morphology and IL-12 gene expression of peritoneal macrophages in mice

目的 探讨放烧复合伤小鼠腹腔巨噬细胞数量、形态及其IL 12基因表达变化的复合效应。方法 于单纯放射损伤 (5Gy)或放烧复合伤 (5Gy合并 12 %II度烧伤 )后不同时相点分离、计数小鼠腹腔巨噬细胞 ,观察其形态学变化 ,RT PCR方法检测其IL 12、p35和p40的基因表达水平。结果 ①同放射损伤组相比 ,放烧复合伤组早期巨噬细胞数量减少速度更快 ,减少程度更大 ,但后期恢复速度明显加快 ;②同放射损伤组相比 ,放烧复合伤组伤后早期溶解坏死的巨噬细胞较多 ,但在形态上更接近正常 ;③两伤组p35和p40伤后表达的变化趋势一致 ,但放烧复合伤组伤后各时相点两基因的表达量明显高于放射损伤组。结论 放烧复合伤在免疫细胞数量、形态和功能上具有“早期加重 ,后期减轻”的复合效应特点 ,提示“早期救治”在放烧复合伤救治中的重要性。

Objective\ To investigate the effect of combined radiation\|burn injury on cell number,morphology and IL 12 gene expression of peritoneal macrophages (pMΦs) in mice. Methods\ At different time points after combined radiation\|burn injury(CRBI) or radiation injury(RI) pMΦs of mice were purified,counted and observed underlight microscope,and the IL 12 transcriptive level was measured with RT\|PCR. Results\ ①Compared with RI group,pMΦs decreased more quickly and seriously at early stage,but recovered better at late stage in CRBI group;②Compared with the irradiation group,there was more necrocytosis in early stage in the CRBI group,but pMΦs in the CRBI group looked closer to normal;③IL 12 p35 transcription levels were suppressed while that of p40 elevated in both CRBI and RI groups. However the transcriptive levels of p35 and p40 was higher in the CRBI group those in the RI group. Conclusion\ The effect of combined radiation\|burn injury on cell number,morphology and IL 12 gene expression of pMΦs are characterized as `worsening at early stage,lightening at late stage',implying the significance of early treatment of CRBI.\;