极低频磁场辐照对细胞间隙连接通讯功能的作用及其阈值研究

Effect and its threshold of ELF magnetic fields on gap junctional intercellular communication

目的 探讨极低频 (ELF)磁场可能存在的促癌或协同促癌作用。方法 应用光漂白后的恢复技术 ,观察ELF磁场对细胞间隙连接通讯 (GJIC)的作用及其阈值。结果 0 4mT及以上强度的ELF磁场辐照能明显抑制细胞GJIC功能 ;0 2mT及以上强度的ELF磁场单独虽无抑制GJIC的效应 ,但可增强TPA所诱导的GJIC的抑制 ;0 1mT的ELF磁场既无单独抑制作用 ,也无协同TPA的作用。

Objective\ To explore the relationship between extremely low frequency (ELF) magnetic fields and carcinogenesis. Methods\ The fluorescence recovery after photobleaching (FRAP) technique was used to determine the effect of ELF magnetic fields and its threshold on gap junctional intercellular communication (GJIC). Results\ FRAP analysis indicated that 0.4 mT ELF or more could inhibit GJIC,and 0.2 mT ELF or more could enhance the inhibition of GJIC by TPA. Conclusion\ ELF magnetic fields might act as a cancer promoter or work in synergy with other cancer promoters.